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The latter has been interpreted as an indication of disrupted NVC (Staines et al., 2018

"Regulation of Gamma-Glutamyl-Cysteine Synthetase by Nonallosteric Feedback Inhibition." Journal of Biological Chemistry , 250(4), 1422-1426

AbaSI-coupled sequencing [121] uses the restriction endonuclease AbaSI that selectively binds 5gmCs, but not 5mCs or Cs and generates double-stranded DNA breaks at the 3 end of binding sites